Switching cryopreservation media between development and clinical manufacturing means revalidating freeze-thaw performance. This data sheet covers a low-viscosity freezing medium with 10% DMSO available in both RUO and GMP grades, so conditions established early carry forward without reformulation. Compared against an industry reference across four iPSC lines and one embryonic stem cell line, post-thaw SSEA4, OCT4, and SOX2 expression remains above the 70% threshold with no significant difference in viability, recovery, doubling time, or expansion fold. NK cell recovery persists through 72 hours post-thaw, and differential scanning calorimetry shows matched melting point and glass transition.
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